Figure 2. Phylogenetic placement of fungi cultivated by Mycetagroicus cerradensis. Left: Overview of phylogenetic relationships among fungi cultivated by attine ants, with fungal lineages associated with ants depicted in black and free-living fungal lineages in grey (adapted from Mikheyev et al. 2010). Middle: (“Global analysis”) Phylogeny of lower-attine cultivars (“G3” sensu Chapela et al. 1994) and closely related free-living fungi reconstructed using the internal transcribed spacer (ITS) rDNA region. The most likely tree with support values from 100 bootstrap replicates above each node is shown. Fungi cultivated by attine ant species are shown in bold (indicated by name of ant host) and free-living fungi in normal font. Lower-attine Clade 1 and Clade 2 cultivars are named as in Mueller et al. (1998). Right: (“Focal analysis”) Phylogeny of Clade 2 lower-attine cultivars and closely related free-living fungi using the ITS region. Shown here is the most likely tree with support values from 1000 bootstrap replicates. Fungi cultivated by attine ant species are shown in bold (indicated by name of ant host) and free-living fungi in normal font. Fungus cultivated by Mycetagroicus cerradensis is outlined with a black box.