Cloning and characterization of a putative farnesoic acid Omethyltransferase gene from the brown planthopper, Nilaparvata lugens
1Key Laboratory of Monitoring and Management of Plant Disease and Insect, Ministry of Agriculture, Nanjing
Agricultural University, Nanjing 210095, China
2The Service Center of Technology, Changzhou Entry-Exit Inspection and Quarantine Bureau, Changzhou
213022, China
3Rice Technology Research and Development Center, China National Rice Research Institute, Hangzhou
310006, China
Abstract
Juvenile hormone (JH) plays key roles in both metamorphosis and adult reproductive processes. Farnesoic acid O-methyltransferase (FAMeT) is thought to be an important enzyme in the JH biosynthetic pathway, catalyzing methylation of farnesoic acid (FA) to methyl farnesoate (MF). A full-length cDNA (NlFAMeT) encoding a 299 amino acid putative FAMeT was isolated from the brown planthopper, Nilaparvata lugens (Stal) (Hemiptera: Geometroidea), a major rice pest in many parts of Asia. NlFAMeT showed high amino acid identities (52-54%) with other insect FAMeTs. Although the NlFAMeT transcript was expressed highly in corpus allatum (CA) and brain (without CA), no correlation was found between NlFAMeT transcript and JH titers. Although only a low level of NlFAMeT transcript was detected in the ovary, a high level was found in the abdomen and should be in one or more tissues undefined in the abdomen. Also, NlFAMeT transcript had a positive change during the vitellogenesis in female adults. These data indicated that NlFAMeT might not be a key enzyme in JH synthesis in N. lugens, but that it may play an important role in the ovary development. It might also be important in some unknown process in a so-far unidentified tissue in the abdomen.
Keywords: juvenile hormone synthesis, mRNA levels, ovary development
Abbreviations: FAMeT farnesoic acid O-methyltransferase; FA, farnesoic acid; MF, methyl farnesoate; CA, corpus
allatum; JH, juvenile hormone; SAM, Sadenosyl-L-methionine; FBP farnesyl biphosphate; PCR, polymerase chain
reaction; qRT-PCR, Quantitative real-time reverse transcriptase polymerase chain reaction; RACE, rapid amplification of cDNA ends
Correspondence:
aheartsun2199@gmail.com,
bzhangchw@jsciq.gov.cn,
cybjhz@yahoo.com.cn,
d2007102089@njau.edu.cn,
e*jemunson@njau.edu.cn, *Corresponding author
Associate Editor: Guy Smagghe was editor of this paper.
Received: 22 August 2008 | Accepted: 10 July 2009 | Published: 13 July 2010
Copyright: This is an open access paper. We use the Creative Commons Attribution 3.0 license that permits unrestricted use, provided that the paper is properly attributed.
ISSN: 1536-2442 | Volume 10, Number 103
Liu S, Zhang C, Yang B, Gu J, Liu Z. 2010. Cloning and characterization of a putative farnesoic acid O-methyltransferase gene from the brown planthopper, Nilaparvata lugens. Journal of Insect Science 10:103, available online: insectscience.org/10.103
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